Fig. 1. Total protein and amino acids contents in C₂C₁₂ myotubes cultivated in various glutamine concentrations (0, 2, 8 or 16 mM) for 48 hours. (A) Total protein and (B) total amino acids (Sum of Leu, Iso, Val, Phe, Thr, Trp, His, Met, Tyr, Lys, Ser, and Gly) contents. The experimental groups are: (0 mM) no glutamine addition in the medium; 2 mM (reference condition), 8 mM or 16 mM L-glutamine concentration in the medium. We presented the findings as to the mean ± SEM of three independent experiments. Results were analyzed using one-way ANOVA and Tukey post-test. (^###p<0.001) as compared with 2 mM glutamine.